![SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein](https://cdn.numerade.com/ask_images/9ba1accee44043f5a7c0785c83c65d99.jpg)
SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein
![SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a](https://cdn.numerade.com/ask_images/5a4ffabce02148148b3f734b19046440.jpg)
SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a
![Cloning of the IFNα8 gene into the recipient vector pET28-TevH by the... | Download Scientific Diagram Cloning of the IFNα8 gene into the recipient vector pET28-TevH by the... | Download Scientific Diagram](https://www.researchgate.net/publication/259607814/figure/fig1/AS:392594883399680@1470613322184/Cloning-of-the-IFNa8-gene-into-the-recipient-vector-pET28-TevH-by-the-Transfer-PCR-TPCR.png)